Degradation of Bunker C Fuel Oil by White-Rot Fungi in Sawdust Cultures Suggests Potential Applications in Bioremediation

نویسندگان

  • Darcy Young
  • James Rice
  • Rachael Martin
  • Erika Lindquist
  • Anna Lipzen
  • Igor Grigoriev
  • David Hibbett
  • Lucía Ramírez
چکیده

Fungal lignocellulolytic enzymes are promising agents for oxidizing pollutants. This study investigated degradation of Number 6 "Bunker C" fuel oil compounds by the white-rot fungi Irpex lacteus, Trichaptum biforme, Phlebia radiata, Trametes versicolor, and Pleurotus ostreatus (Basidiomycota, Agaricomycetes). Averaging across all studied species, 98.1%, 48.6%, and 76.4% of the initial Bunker C C10 alkane, C14 alkane, and phenanthrene, respectively were degraded after 180 days of fungal growth on pine media. This study also investigated whether Bunker C oil induces changes in gene expression in the white-rot fungus Punctularia strigosozonata, for which a complete reference genome is available. After 20 days of growth, a monokaryon P. strigosozonata strain degraded 99% of the initial C10 alkane in both pine and aspen media but did not affect the amounts of the C14 alkane or phenanthrene. Differential gene expression analysis identified 119 genes with ≥ log2(2-fold) greater expression in one or more treatment comparisons. Six genes were significantly upregulated in media containing oil; these genes included three enzymes with potential roles in xenobiotic biotransformation. Carbohydrate metabolism genes showing differential expression significantly accumulated transcripts on aspen vs. pine substrates, perhaps reflecting white-rot adaptations to growth on hardwood substrates. The mechanisms by which P. strigosozonata may degrade complex oil compounds remain obscure, but degradation results of the 180-day cultures suggest that diverse white-rot fungi have promise for bioremediation of petroleum fuels.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The potential for white-rot fungi in the treatment of pollutants

Lignin-degrading white-rot fungi have the unique ability to degrade/mineralize a broad spectrum of structurally diverse toxic environmental pollutants. Extracellular peroxidases are important in degrading some, but not all, xenobiotic compounds. More research is needed to realize the potential of white-rot fungi in field-scale applications. Recent progress in our knowledge of the biochemistry a...

متن کامل

Field scale bioremediation of tar contaminated soil with commercial mushroom PJeurotus ostreatus refuse

Pollution of soils with poiycyciic aromatic andheteroaromatic compounds (PACS) from various uses of oil products has impontant economic and toxicological implications for the polluted areas. The larger PACS are quite refractory to microbial degradation. Due to the ability of the fimgal ligninolytic enzyme systems to also degrade a range of environmental pollutants, e.g. PACS,white rot fimgihave...

متن کامل

Bioremediation and Detoxification of the Textile Wastewater with Membrane Bioreactor Using the White-rot Fungus and Reuse of Wastewater

Background: Application of membrane technology to wastewater treatment has expanded over the last decades due to increasingly stringent legislation, greater opportunities for water reuse/recycling processes and continuing advancement in membrane technology. Objectives: In the present study, a bench-scale submerged microfiltration membrane bioreactor (MBR) was used to assess the treatment of tex...

متن کامل

Lignin degradation and roles of white rot fungi: Study on an efficient symbiotic system in fungus-growing termites and its application to bioremediation

White rot fungi produce extracellular phenoloxidases and can decompose lignin efficiently. Elfvingia applanata has been successfully applied for the bioconversion of bisphenol A, suggesting the usefulness of white rot fungi for bioremediation. In order to attain real bioremediation, the recycling of ecomolecules in the ecosystem is important. The sophisticated symbiotic system of white rot fung...

متن کامل

Uses of mushrooms in bioremediation: A review

One of the major environmental problems facing the world today is the contamination of soil, water and air by toxic chemicals as a result of industrialization and extensive use of pesticides in agriculture. Incineration is currently the most effective and common remediation practice but is costly in terms of money and energy used. A rapid cost effective and ecologically responsible method of cl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2015